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1.
2022 IEEE 14th International Conference on Humanoid, Nanotechnology, Information Technology, Communication and Control, Environment, and Management, HNICEM 2022 ; 2022.
Article in English | Scopus | ID: covidwho-20240848

ABSTRACT

Mental health is more than the presence or absence of a mental illness. COVID-19 has led to self and social isolation, disconnection from family and friends. More people than ever are experiencing feelings of helplessness, isolation, grief, anxiety, and depression. In such cases, the team took advantage of the opportunity to raise mental health awareness by developing a trilogy of interactive short films with a website that would support the project's purpose. The film has three characters with different stories, namely Marion;a college student who is struggling to adjust to his new average experience from his online class and Kayla;a nurse who is also a mother of three children, who must participate in treating the patients with COVID at the hospital, and Marie;an OFW who works from Saudi Arabia wants to go home to visit her family. The study also intends to explore the impact of coping strategies and perceived social support on one's ability to handle social isolation stress. Each of the characters' stories will represent the different outlook on possible mental health symptoms that everyone has experienced during the pandemic. The stories presented in the film will be based on real-life circumstances and experiences of people who have lived through the pandemic. The story aims to give hope to its viewers on what lies ahead of the pandemic. Upon testing the produced output, the study results show that most people only have a general idea when it comes to the topic of mental health issues. The study also yielded that the project has spread and increased awareness about the topic under study. Although the project could attain its specific goals and adjectives, the best and only conclusion is to seek professional help from credible and trusted experts in their respective health fields. © 2022 IEEE.

2.
Zhonghua Yu Fang Yi Xue Za Zhi ; 57: 1-14, 2023 May 17.
Article in Chinese | MEDLINE | ID: covidwho-2327395

ABSTRACT

During the global efforts to prevent and control the COVID-19 pandemic, extensive research and development of SARS-CoV-2 vaccines using various technical approaches have taken place. Among these, vaccines based on adenovirus vector have gained substantial knowledge and experience in effectively combating potential emerging infectious diseases, while also providing novel ideas and methodologies for vaccine research and development (R&D). This comprehensive review focuses on the adenovirus vector technology platform in vaccine R&D, emphasizing the importance of mucosal immunity induced by adenoviral vector-based vaccine for COVID-19 prevention. Furthermore, it analyzes the key technical challenges and obstacles encountered in the development of vaccines based on the adenovirus vector technology platform, with the aim of providing valuable insights and references for researchers and professionals in related fields.

3.
Medical Journal of Wuhan University ; 44(3):253-260, 2023.
Article in Chinese | Scopus | ID: covidwho-2320844

ABSTRACT

Objective: To investigate the incidence rates of anxiety and depression among the COVID-19 patients and their association with clinical features and laboratory variables. Methods: A total of 371 COVID-19 patients were recruited from Wuhan Leishenshan Hospital from Jan 20 to May 10, 2020. The anxiety and depression were assessed by using the Hosptial Anxiety and Depression Score (HAD), the Self-rating Anxiety Scale (SAS), and Self-rating Depression Scale (SDS). The clinical features and laboratory variables were collected through electronic medical record. Statistical analyses were used to investigate the influence factors associated with anxiety and depression. Results: Among the 371 COVID-19 patients, the frequency of anxiety measured by HAD or SAS was 22. 91% and 24. 26%, respectively. The frequency of depression based on HAD or SDS was 16. 17% and 9. 43%, respectively. There were more female unmarried individuals in the anxiety or depression group. Anxiety or depression scores were significantly inversely correlated with the time for nucleic acid test turning negative. D-dimer and interleukin-6 (IL-6) were significantly elevated in the individuals with anxiety and depression. Statistically significant downregulations of lymphocyte counts, hemoglobin, and creatinine were found in anxiety and depression group. There was a negative association between creatinine and anxiety or depression scores. One unit upregulation of IL-6 and downregula-tion of lymphocyte counts could increase the hazard odds ratio of anxiety or depression by 10. 7% and 68. 9%, respectively. Conclusion: The COVID-19 patients with anxiety or depression symptoms had several different clinical features and laboratory findings in comparison with the patients without, which could lead to a poor prognosis of this disease. Clinicians should pay more attention to these indicators for anxiety or depression. Targeted psychological interventions should be implemented to minimize the negative impact of the psychological burden and to improve the quality life and disease outcome. © 2023 Editorial Board of Medical Journal of Wuhan University. All rights reserved.

4.
Chinese Journal of Practical Nursing ; 38(30):2321-2326, 2022.
Article in Chinese | Scopus | ID: covidwho-2320553

ABSTRACT

In the context of normalized prevention of COVID- 19 epidemic and the in- depth promotion of building a healthy China, the consensus on integrating humanistic care into nursing work is taking root in the hearts of the people. As the guardian of people′s life and health and the main force in promoting the construction of a healthy China, the implementation of humanistic care by nurses in clinical work is crucial to improving the quality of nursing, improving patient outcomes, and promoting a harmonious relationship between nurses and patients. The acceleration of the current medical system reform process and the rapid release of the people′s demand for health services have further highlighted the urgency of improving the humanistic care capacity of nursing. This article focuses on improving nurses′ awareness, professional knowledge and skills of humanistic care, strengthening exploration and practice of nursing humanistic care management, focusing on creating a campus environment and culture of humanistic care for students, intensifying theoretical research on nursing humanistic care, integrating humanistic care into smart nursing, caring for nurses′ own development and physical and mental health, etc., will provide ideas for improving the humanistic care ability, helping the high-quality development of nursing, and promoting the construction of a healthy China. © Osmani F., 2023.

5.
Chinese Journal of Clinical Infectious Diseases ; 13(1):9-15, 2020.
Article in Chinese | EMBASE | ID: covidwho-2305597

ABSTRACT

Objective: To compare the efficacy of the combination of abidol, lopinavir/ritonavir plus recombinant interferon alpha-2b (rIFNalpha-2b) and the combination of lopinavir/ritonavir plus rIFNalpha-2b for patients with COVID-19 in Zhejiang province. Method(s): A multicenter prospective study was carried out to compare the efficacy of triple combination antiviral therapy and dual combination antiviral therapy in 15 medical institutions of Zhejiang province during January 22 to February 16, 2020. All patients were treated with rIFNalpha-2b (5 million U, 2 times/d) aerosol inhalation, in addition 196 patients were treated with abidol (200 mg, 3 times/d) + lopinavir/ritonavir (2 tablets, 1 time/12 h) (triple combination group) and 41 patients were treated with lopinavir/ritonavir (2 tablets, 1 time/12 h) (dual combination group). The patients who received triple combination antiviral therapy were further divided into three subgroups: <48 h, 3-5 d and >5 d according the time from the symptom onset to medication starting. The therapeutic efficacy was compared between triple combination group and dual combination group, and compared among 3 subgroups of patients receiving triple combination antiviral therapy. SPSS 17.0 software was used to analyze the data. Result(s): The virus nucleic acid-negative conversion time in respiratory tract specimens was (12.2+/-4.7) d in the triple combination group, which was shorter than that in the dual combination group [(15.0+/-5.0) d] (t=6.159, P<0.01). The length of hospital stay in the triple combination group [12.0 (9.0, 17.0) d] was also shorter than that in the dual combination group [15.0 (10.0, 18.0) d] (H=2.073, P<0.05). Compared with the antiviral treatment which was started within after the symptom onset of in the triple combination group, the time from the symptom onset to the viral negative conversion was 13.0 (10.0, 17.0), 17.0 (13.0, 22.0) and 21.0 (18.0, 24.0) d in subgroups of 48 h, 3-5 d and >5 d, respectively (Z=32.983, P<0.01), while the time from antiviral therapy to viral negative conversion was (11.8+/-3.9), (13.5+/-5.1) and (11.2+/-4.3) d, respectively(Z=6.722, P<0.05). Conclusion(s): The triple combination antiviral therapy of abidol, lopinavir/litonavir and rIFNalpha-2b shows shorter viral shedding time and shorter hospitalization time, compared with the dual combination antiviral therapy;and the earlier starting triple combination antiviral therapy will result in better antiviral efficacy.Copyright © 2020 by the Chinese Medical Association.

6.
Advances in Health and Disease ; 62:225-247, 2023.
Article in English | Scopus | ID: covidwho-2267343

ABSTRACT

Superior mesenteric venous (SMV) thrombosis is an infrequent thrombotic event of hypercoagulable states that can cause intestinal ischemia, hemorrhage, or infarction. Despite severe consequences, SMV thrombosis often presents with nonspecific clinical features, including abdominal pain, distension, diarrhea, nausea, vomiting, intestinal obstruction, and bloody stool. SMV thrombosis may cause portal hypertension and its associated symptoms like intractable ascites of profuse production. Laboratory data may include elevation of hepatic enzyme, amylase, or lipase levels. SMV thrombosis is associated with a specific etiology (malignancy, thrombophilia, inflammatory bowel disease, intra-abdominal infection, or surgery). Other conditions or diseases such as pregnancy, puerperium, influenza, coronavirus disease-2019, and vaccine-induced immune thrombocytopenia are associated with thrombosis formation in various vessels, including SMV. Computed tomography (CT) imaging in the venous phase is considered the most reliable diagnostic method of choice. CT imaging may find acute thrombi present in SMV associated with submucosal edema in some intestinal loops. Management strategies are complicated by an underlying prothrombotic state and an increased risk of bleeding. SMV thrombosis can be reversed by effective anticoagulation. Prompt transcatheter thrombolysis can achieve early SMV revascularization. Early diagnosis prevents anticoagulation with continuous intravenous infusion of unfractionated heparin preventing subsequent consequences. Open laparotomy with mesenteric revascularization and resection of necrotic bowel has been considered the gold standard of care. Despite systemic anticoagulation therapy with intravenous heparin, the decision of an exploratory open thrombectomy could be made for patient deterioration clinically. The mortality rate among patients with acute SMV thrombosis ranges from less than 10% to 50%, depending on disease severity requiring surgical intervention. Negative predictors of survival include a higher Charlson comorbidity index and malignancy. Earlier diagnosis by the widespread use of CT scanning and aggressive treatment with anticoagulation may prevent progression to gangrenous bowel and lower mortality rates. © 2023 Nova Science Publishers, Inc. All rights reserved.

7.
Chinese Journal of Clinical Infectious Diseases ; 13(1):9-15, 2020.
Article in Chinese | EMBASE | ID: covidwho-2286480

ABSTRACT

Objective: To compare the efficacy of the combination of abidol, lopinavir/ritonavir plus recombinant interferon alpha-2b (rIFNalpha-2b) and the combination of lopinavir/ritonavir plus rIFNalpha-2b for patients with COVID-19 in Zhejiang province. Method(s): A multicenter prospective study was carried out to compare the efficacy of triple combination antiviral therapy and dual combination antiviral therapy in 15 medical institutions of Zhejiang province during January 22 to February 16, 2020. All patients were treated with rIFNalpha-2b (5 million U, 2 times/d) aerosol inhalation, in addition 196 patients were treated with abidol (200 mg, 3 times/d) + lopinavir/ritonavir (2 tablets, 1 time/12 h) (triple combination group) and 41 patients were treated with lopinavir/ritonavir (2 tablets, 1 time/12 h) (dual combination group). The patients who received triple combination antiviral therapy were further divided into three subgroups: <48 h, 3-5 d and >5 d according the time from the symptom onset to medication starting. The therapeutic efficacy was compared between triple combination group and dual combination group, and compared among 3 subgroups of patients receiving triple combination antiviral therapy. SPSS 17.0 software was used to analyze the data. Result(s): The virus nucleic acid-negative conversion time in respiratory tract specimens was (12.2+/-4.7) d in the triple combination group, which was shorter than that in the dual combination group [(15.0+/-5.0) d] (t=6.159, P<0.01). The length of hospital stay in the triple combination group [12.0 (9.0, 17.0) d] was also shorter than that in the dual combination group [15.0 (10.0, 18.0) d] (H=2.073, P<0.05). Compared with the antiviral treatment which was started within after the symptom onset of in the triple combination group, the time from the symptom onset to the viral negative conversion was 13.0 (10.0, 17.0), 17.0 (13.0, 22.0) and 21.0 (18.0, 24.0) d in subgroups of 48 h, 3-5 d and >5 d, respectively (Z=32.983, P<0.01), while the time from antiviral therapy to viral negative conversion was (11.8+/-3.9), (13.5+/-5.1) and (11.2+/-4.3) d, respectively(Z=6.722, P<0.05). Conclusion(s): The triple combination antiviral therapy of abidol, lopinavir/litonavir and rIFNalpha-2b shows shorter viral shedding time and shorter hospitalization time, compared with the dual combination antiviral therapy;and the earlier starting triple combination antiviral therapy will result in better antiviral efficacy.Copyright © 2020 by the Chinese Medical Association.

8.
Journal of Immigrant and Refugee Studies ; 21(1):15-27, 2023.
Article in English | Scopus | ID: covidwho-2246631

ABSTRACT

Based on 25 in-depth interviews collected during the COVID pandemic from Chinese academic immigrants in the U.S., we find that COVID immediately halted their transnational travels. Furthermore, catalyzed by changes in the Sino-U.S. geopolitical relationship, the soaring Anti-Asian hate in the U.S., and the raging storm of patriotism and nationalism in China, COVID impacts academic migrants' perceptions of opportunities, pursuits of transnational movements, and ethnic and diasporic identities. The disrupted transnational migration of people and knowledge due to the intersection of the pandemic, social contexts, and geopolitics may have long-term detrimental effects at the individual, institutional, national, and global levels. © 2023 Taylor & Francis Group, LLC.

9.
Chinese General Practice ; 25(33):4217-4226, 2022.
Article in Chinese | Scopus | ID: covidwho-2145251

ABSTRACT

Background Mental health problems among college students have become increasingly prominent. Social anxiety is one of the prevalent psychological problems among college students. Objective To explore the research hot spots,frontiers and trends on social anxiety among college students,and provide reference for researchers participating in the research of college students' social anxiety in the future. Methods 643 English articles in database of Web of Science(WOS) and 166 Chinese articles in database of China National Knowledge Infrastructure(CNKI)from 2000 to 2021 were analyzed using CiteSpace on August 27,2021. Results The number of English articles on social anxiety among college students showed an increasing trend from 2000 to 2021. The research hot spots and frontiers of social anxiety among college students were mainly focused on substance abuse,mobile phone and internet addiction,negative evaluation fear,racial differences,psychological intervention and COVID-19 epidemic. The future research trends were mainly focused on the mechanism of substance abuse and mobile phone addiction. Conclusion Chinese scholars can refer to the research hot spots,trends and the differences between domestic and foreign research shown by this visual analysis,and focus on the related problems of substance abuse and mobile internet addiction among college students with social anxiety. © 2022 Chinese General Practice. All rights reserved.

10.
Zhonghua Yu Fang Yi Xue Za Zhi ; 56(10): 1395-1400, 2022 Oct 06.
Article in Chinese | MEDLINE | ID: covidwho-2090421

ABSTRACT

In the context of the global pandemic of COVID-19, the epidemic intensity, epidemic characteristics and infection risk of influenza have presented new features. COVID-19 and influenza have simultaneously emerged in many regions of the world. COVID-19 and influenza are similar in terms of transmission mode, clinical symptoms and other aspects. There are also similarities in the mechanism of influenza virus and novel coronavirus on cells. At the same time, it is feasible and significant to do a good job in the prevention and control of COVID-19 and influenza. This paper discusses the relevant strategies and measures for the joint prevention and control of influenza and novel coronavirus from the aspects of influenza vaccination to prevent co-infection, simultaneous vaccination of influenza vaccine and novel coronavirus vaccine, etc., and puts forward corresponding thoughts and suggestions, in order to provide scientific support for the formulation of strategies on seasonal influenza vaccine and novel coronavirus vaccination.


Subject(s)
COVID-19 , Influenza Vaccines , Influenza, Human , Humans , Influenza, Human/prevention & control , Influenza, Human/epidemiology , COVID-19 Vaccines , COVID-19/prevention & control , Seasons , Vaccination , SARS-CoV-2
11.
3rd International Conference on Internet and E-Business, ICIEB 2022 ; : 56-61, 2022.
Article in English | Scopus | ID: covidwho-2088926

ABSTRACT

With the outbreak of external shocks, cash-strapped retailers can order products from suppliers and sell them to meet uncertain demand. We present a mathematical model of a supply chain involving a retailer and a supplier. We use the Newsvendor model with budget constraint and inventory leftovers as the framework and utilize other analytical theories such as the Stackelberg game and early payment discount to model the strategic behavior of suppliers and retailers. Furthermore, for different countries countering COVID-19, our research can speculate each unique case by altering parameters. Under mild assumptions, lower boundary of the demand does not affect the optimal ordering quantity, and it is also worth noting that when the sale price increases, the probability of bankruptcy increases because supplier increase her wholesale price so that retailer makes less profits. Also, when sale price is relatively high, retailer tends to become aggressive on ordering products. Thus, when demand is insufficient, retailer goes bankruptcy. © 2022 ACM.

12.
4th International Conference on Intelligent Control, Measurement and Signal Processing, ICMSP 2022 ; : 748-752, 2022.
Article in English | Scopus | ID: covidwho-2052014

ABSTRACT

In order to prevent COVID-19 effectively, non-contact body temperature measurement and human identification are required in public places, but face recognition based on visible light cannot meet the requirements. Therefore, this paper proposes a thermal imaging face recognition method based on temperature block feature extraction. Histogram equalization and median filter are used to preprocess the face image, and Sobel operator is used for face detection;Six dimensional features including temperature mean, standard deviation and adjacent difference are extracted from each temperature block in the average poolinged temperature matrix, and classified by max-correlation-coefficient method. The experimental results show that the recognition rate of this method is 6.1% higher than that of PCA method with the temperature block size of boldsymbol{2times 2}. When using the same hardware to execute the program, if the two recognition rates are very close, the average test time of the proposed method is 22.2% less than the one of deep learning models such as Alexnet. Furthermore, the proposed method has strong robustness for small training sample set. For example, the recognition rate of single training sample model can reach 0.7, while in the deep learning model, except Mobilenet can reach 0.6, all of the others are less than 0.4. © 2022 IEEE.

13.
23rd International Conference on Enterprise Information Systems, ICEIS 2021 ; 1:183-191, 2021.
Article in English | Scopus | ID: covidwho-2045818

ABSTRACT

This study describes an activity based traffic indicator system to provide information for COVID-19 pandemic management. The activity based traffic indicator system does this by utilizing a social probability model based on the birthday paradox to determine the exposure risk, the probability of meeting someone infected (PoMSI). COVID-19 data, particularly the 7-day moving average of the daily growth rate of cases (7-DMA of DGR) and cumulative confirmed cases of next week covering a period from April to September 2020, were then used to test PoMSI using Pearson correlation to verify whether it can be used as a factor for the indicator. While there is no correlation for the 7-DMA of DGR, PoMSI is strongly correlated (0.671 to 0.996) with the cumulative confirmed cases and it can be said that as the cases continuously rise, the probability of meeting someone COVID positive will also be higher. This shows that indicator not only shows the current exposure risk of certain activities but it also has a predictive nature since it correlates to cumulative confirmed cases of next week and can be used to anticipate the values of confirmed cumulative cases. This information can then be used for pandemic management. Copyright © 2021 by SCITEPRESS – Science and Technology Publications, Lda. All rights reserved.

14.
Neurology Perspectives ; 2(1):9-20, 2022.
Article in English, Spanish | Scopus | ID: covidwho-1959889

ABSTRACT

Background: The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which causes the disease COVID-19, has caused a pandemic that has rapidly affected the whole world and caused a significant threat to public health. The aim of this study was to investigate and analyze the social and occupational effects of the COVID-19 pandemic on patients with multiple sclerosis (MS) in three different countries: China, Spain, and Cuba. Methods: A cross-sectional survey was designed to assess the social and occupational effects of the COVID-19 pandemic in MS patients in these three countries, using a 25-item anonymous online questionnaire, structured into three sections. Quantitative data are expressed as mean (standard deviation), and quantitative data as absolute frequency and percentage. Results: A total of 361 participants responded to the questionnaire: 194 from China, 104 from Spain, and 63 from Cuba. We found no cases of COVID-19 among Chinese patients with MS, and few cases in Spain and Cuba. Respondents reported different levels of impact on relationships with friends, family, and colleagues, and patients in all three countries described increased use of digital or social media platforms. Spanish patients reported a significantly less negative impact than those in Cuba and China. Mental and cognitive effects were similar in all three countries, although China seemed to have a better situation. We also found that the time spent exercising decreased at specific points during the pandemic, but with few changes in dietary habits. Patients reported little or no change in their means of transport in all three countries. Most patients in all three countries reported little or no physical deterioration, especially in Chinese patients (82.47%), compared to the Spanish (70.20%) and Cuban respondents (73.02%). In general, patients from all three countries demonstrated confidence in overcoming the COVID-19 pandemic, although to a lesser extent among Spanish respondents. Conclusions: During the pandemic, family support was more effective in China than in Cuba and Spain. Neither COVID-19 infections nor the number of MS relapses increased significantly during lockdown in any of the three countries. Regarding their economic situation, Spanish MS patients reported a significantly less severe negative impact than those in Cuba and China. Patients from all three countries used digital or social media platforms more frequently, probably to maintain personal relationships. Chinese and Cuban respondents were more confident of the control of the pandemic than the Spanish, who were more pessimistic. © 2021

15.
2nd International Conference on Artificial Intelligence and Computer Engineering, ICAICE 2021 ; : 570-574, 2021.
Article in English | Scopus | ID: covidwho-1948774

ABSTRACT

The sudden COVID-19 pandemic made us feel the limitations of offline sales. In order to let consumers fully realize the convenience of the Internet era and highlight the advantages of online sales, a personalized online florist system (OFS) is developed. The website takes JSP (JavaServer Pages) + SSM (Spring + SpringMVC + MyBatis) framework as the development technology and based on B/S (Browser/Server) structure, an online florist system based on JavaWeb is designed and implemented. © 2021 IEEE.

16.
Swiss Medical Weekly ; 152(SUPPL 258):2S, 2022.
Article in English | EMBASE | ID: covidwho-1913164

ABSTRACT

Post-Covid condition (or long covid) describes the persistence of symp-toms several months to years after SARS-CoV-2 infection. It has been in-creasingly described in children as well, particularly in preteens and teen-agers. Most commonly reported symptoms include fatigue, difficulty con-centrating, sleeping disorders, shortness of breath, and mood disorders. Treatment of these symptoms is limited and consists mostly of adaptation of physical and mental activities, reconditioning and motivational sup-port. Improving information and awareness of this condition are priorities to properly evaluate and treat these children. The Department of General Pediatrics in Geneva has set up a specific consultation for children and adolescents in spring 2021. In addition, in collaboration with the Depart-ment of Community Medicine and Primary Care and Emergency Medicine, we developed an academic digital citizen platform (RAFAEL), which com-bines an information site, a chatbot, and webinars/workshops for both children and adults. We report here our experience with the use of the pediatric chatbot which was launched on February 1st 2022. The chatbot is a conversational agent that can answer questions in real-time and reorient people to a special-ist/advisor if needed. Chatbot technology is useful in providing verified information and prompting communication and interaction with a large number of people simultaneously and can be adapted to all age groups. It is particularly appealing to teenagers who are very familiar with the con-cept of chatbot technology and may find this way to communicate more appealing than regular website information or traditional literature. We will report the initial frequently asked questions and their evolution over time within this chatbot. We will also report the number of users of the pediatric chatbot, the overall and individual match rates, and users' satis-faction. A platform, such as RAFAEL, could become a new way to interact with young patients regarding non urgent subjects, prevention, chronic dis-eases, as well as a way to use their regular feed-back to be as close as possible to the patients' needs.

17.
Chinese Journal of Laboratory Medicine ; 45(4):393-398, 2022.
Article in Chinese | Scopus | ID: covidwho-1911767

ABSTRACT

Objective To study the difference in the extraction efficiency of the novel coronavirus (2019-nCoV) nucleic acid by using magnetic beads method, centrifugal column method and one-step method. Methods On March 5, 2021, 10 throat swabs were collected from the staff working in the nucleic acid sampling room in Department of Clinical Laboratory, Affiliated Taikang Xianlin Drum Tower Hospital, Medical School of Nanjing University. The positive quality control samples were mixed into the swabs and used as mock positive samples. The RNA was extracted from simulated positive samples and their diluted samples by using magnetic beads method, centrifugation column method and one-step method. The purity (A260/A280 ratio) and concentration of the nucleic acid obtained were measured by micro-uv photometry, and fluorescence quantitative PCR was performed to compare the CT value and extraction efficiency. The three methods were used to extract the simulated weak positive specimens and to compare the difference of CT values after amplification. The measurement data that followed normal distribution were expressed by x ± s, the t test was used for comparing in the same group, and single factor analysis of variance was used for comparing among multiple groups. A P value smaller than 0.05 indicated a significant difference. Results 2019-nCoV nucleic acid extracted by magnetic bead method, centrifugal column method and one-step method could amplify positive results. There was no significant difference between the CT value of RNA amplification extracted by magnetic bead method and one-step method (t=- 0.995, P=0.376). The CT values of orf1ab gene amplified by centrifugal column method, magnetic bead method and one-step method were 29.28±0.06, 30.82±0.14 and 29.79±0.01 respectively (F=11.196, P=0.041). The CT values of E gene were 28.52±0.40, 27.33±0.78 and 27.38±0.13 respectively (F= 3.407, P=0.169). The CT values of N gene were 28.61±1.02, 27.24±0.20 and 27.25±0.47, respectively (F=2.880, P=0.020). The CT values of human genes extracted by centrifugal column method, magnetic bead method and one-step method were 19.68±0.36, 20.14±0.06 and 20.58±0.49 respectively, which was statistically significant (F=4.904, P=0.048). The CT value of amplified human gene was affected by the dilution of human samples twice. The CT value of undiluted samples was smaller than that of diluted samples twice, with a difference of 2.95±0.22, which was statistically significant (t=-3.025, P=0.039). The extraction time of one-step method, magnetic bead method and centrifugal column method were (15.00±1.50), (20.00±1.50) and (40.00±5.5) min respectively, and the difference was statistically significant (F=688, P=0.027). Conclusions Magnetic bead method, centrifugal column method and one-step method can be used to extract 2019-nCoV nucleic acid, for the centrifugal column method has a higher extraction efficiency than the magnetic bead method and the one-step method. The one-step method is the fastest, followed by the magnetic bead method and the centrifugal column method. A large number of clinical samples can be processed using the magnetic bead method and one-step method. One-step rapid nucleic acid test can also be performed on samples from emergency and fever clinics. It is not recommended to dilute specimens for testing. In order to improve the detection rate, extracting RNA from highly suspected samples with negative initial nucleic acid test by centrifugal column method is suggested. © 2022 Chinese Medical Journals Publishing House Co.Ltd. All rights reserved.

19.
17th International Conference on Web Information Systems and Technologies (WEBIST) ; : 250-256, 2021.
Article in English | English Web of Science | ID: covidwho-1884608

ABSTRACT

This study describes an activity based traffic indicator system to provide information for the management of the COVID-19 pandemic. The purpose of the indicator is to be able to discern what activities (e.g., grocery shopping and sports) are dangerous, uncertain, or safe to do in the Philippines on a per-region basis through a traffic light's color of red, yellow, and green. The activity based traffic indicator system does this by utilizing a social probability model based on the birthday paradox to determine the exposure risk which is the probability of meeting someone infected (PoMSI). Additionally, a website called SANITTISE was created to host the indicator system and also to display other pandemic related graphs. Furthermore, a user interface/user experience (UI/UX) test was conducted through a survey to measure the effectiveness of the website created. Regarding the results of the test, it was positive since all of the sections were well received in the survey. This meant that the work done on the website appears substantial as the respondents were able to understand the contents and purpose of the website and also effectively traverse the website and create deductions from the information available on the website.

20.
Current Issues in Tourism ; : 17, 2022.
Article in English | Web of Science | ID: covidwho-1868183

ABSTRACT

This research aims to grasp the evolution of consumer demand and improve the resilience of the hotel industry under the public health crisis (COVID-19). Online reviews of 7,679 hotels in 10 cities were collected from Ctrip, China's major online hotel platform. Then, we applied opinion mining and time evolution to mine the change in consumer demand before, during, and after the COVID-19 pandemic. Findings show that some consumer demands (e.g. epidemic safety) will change during the outbreak period. However, during the nonoutbreak period, users were more concerned about their own check-in experience (e.g. hotel facilities, front desk services). This article provides new ideas for identifying the dynamic value of online reviews. We suggest that businesses focus on ensuring hotel safety during the crisis period. The results contribute essential theoretical and practical significance to the hotel industry's crisis management during public health crises.

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